>>Appendix - One-Phor-All Buffer PLUS activity guide
The table that follows is a guide to the activity of some of our restriction enzymes in One-Phor-All Buffer PLUS (OPA+). Enzymes not listed in the table have not been tested for activity in OPA+ buffer. For each enzyme in the table the following pieces of information are provided:
The optimum concentration of OPA+ buffer - the concentration at which the enzyme's activity is highest (the buffer is a 10× concentrate1).
The enzyme's activity in the optimum concentration of OPA+ buffer as a percentage of its activity in the assay buffer listed on the Certificate of Analysis.
Percentages are designated as follows: ++ = 50-99%; +++ = 100%; ++++ = 101-199%; +++++ > 200%
|
Restriction enzyme
|
Optimum OPA
buffer conc.
|
Activity in OPA
as a percentage of activity in assay buffer
| |
Aat II
|
1×
|
+++++
| |
Acc I
|
1×
|
++
| |
Alu I
|
1×
|
+++
| |
Apa I
|
0.5×
|
+++++
| |
Ava I
|
2×
|
+++
| |
Ava II
|
1×
|
+++++
| |
Bam H I
|
1×
|
++++
| |
Bgl I2
|
1×
|
++
| |
Bgl II
|
1×
|
+++
| |
Cla I
|
1×
|
+++++
| |
Dde I
|
1×
|
+++++
| |
Dra I
|
1×
|
+++
| |
EcoR I3
|
1×
|
+++
| |
EcoR V
|
2×
|
+++
| |
Hae III
|
1×
|
+++
| |
Hha I
|
1×
|
+++
| |
Hinc II
|
1×
|
+++
| |
Hind III
|
1×
|
+++
| |
Hinf I
|
1×
|
+++++
| |
Hpa I
|
1×
|
+++
| |
Hpa II
|
1×
|
+++
| |
Kpn I
|
1×
|
+++
| |
Mlu I
|
2×
|
+++
| |
Msp I
|
1×
|
+++
| |
Nar I
|
1×
|
+++
| |
Nco I 3
|
1×
|
++++
| |
Nde I
|
2×
|
++
| |
Nhe I
|
1×
|
+++
| |
Not I4
|
2×
|
++++
| |
Pst I
|
1×
|
+++++
| |
Pvu I
|
2×
|
++++
| |
Pvu II5
|
1×
|
+++
| |
Rsa I
|
1×
|
+++++
| |
Sac I
|
1×
|
+++
| |
Sac II
|
1×
|
++
| |
Sal I
|
2×
|
++
| |
Sau3A I
|
1×
|
+++
| |
Sca I
|
2×
|
+++++
| |
Sfi I
|
1×
|
+++
| |
Sma I
|
1×
|
+++
| |
Sph I
|
1×
|
+++
| |
Stu I
|
1×
|
+++
| |
Taq I
|
1×
|
++++
| |
Xba I
|
1×
|
+++
| |
Xho I
|
1×
|
+++++
|
1 OPA+ buffer (27-0901-02) will be provided free of charge upon request. Please inquire.
2 With Bgl I, addition of KCl to a final concentration of 100 mM KCl is required.
3 With EcoR I and Nco I, star activity may be observed in 1× OPA+ when DNA is digested for longer than 1 hour. Star activity can be eliminated during extended incubations by using 2× concentration.
4 With Not I, addition of Triton X-100 to a final concentration of 0.01% (w/v) is required.
5 With Pvu II, addition of NaCl to a final concentration of 50 mM is required.
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